Transforming pollution into solutions: A bibliometric analysis and sustainable strategies for reducing indoor microplastics while converting to value-added products.

Microplastics (MPs), as emerging indoor contaminants, have garnered attention due to their ubiquity and unresolved implications for human health. These tiny particles have permeated indoor air and water, leading to inevitable human exposure. Preliminary evidence suggests MP exposure could be linked to respiratory, gastrointestinal, and potentially other health issues, yet the full scope of their effects remains unclear. To map the overall landscape of this research field, a bibliometric analysis based on research articles retrieved from the Web of Science database was conducted. The study synthesizes the current state of knowledge and spotlights the innovative mitigation strategies proposed to curb indoor MP pollution. These strategies involve minimizing the MP emission from source, advancements in filtration technology, aimed at reducing the MP exposure. Furthermore, this research sheds light on cutting-edge methods for converting MP waste into value-added products. These innovative approaches not only promise to alleviate environmental burdens but also contribute to a more sustainable and circular economy by transforming waste into resources such as biofuels, construction materials, and batteries. Despite these strides, this study acknowledges the ongoing challenges, including the need for more efficient removal technologies and a deeper understanding of MPs' health impacts. Looking forward, the study underscores the necessity for further research to fill these knowledge gaps, particularly in the areas of long-term health outcomes and the development of standardized, reliable methodologies for MP detection and quantification in indoor settings. This comprehensive approach paves the way for future exploration and the development of robust solutions to the complex issue of microplastic pollution.

Uncovering environmental performance patterns of plastic packaging waste in high recovery rate countries: An example of EU-27.

Plastic consumption and its end-of-life management pose a significant environmental footprint and are energy intensive. Waste-to-resources and prevention strategies have been promoted widely in Europe as countermeasures; however, their effectiveness remains uncertain. This study aims to uncover the environmental footprint patterns of the plastics value chain in the European Union Member States (EU-27) through exploratory data analysis with dimension reduction and grouping. Nine variables are assessed, ranging from socioeconomic and demographic to environmental impacts. Three clusters are formed according to the similarity of a range of characteristics (nine), with environmental impacts being identified as the primary influencing variable in determining the clusters. Most countries belong to Cluster 0, consisting of 17 countries in 2014 and 18 countries in 2019. They represent clusters with a relatively low global warming potential (GWP), with an average value of 2.64 t CO2eq/cap in 2014 and 4.01 t CO2eq/cap in 2019. Among all the assessed countries, Denmark showed a significant change when assessed within the traits of EU-27, categorised from Cluster 1 (high GWP) in 2014 to Cluster 0 (low GWP) in 2019. The analysis of plastic packaging waste statistics in 2019 (data released in 2022) shows that, despite an increase in the recovery rate within the EU-27, the GWP has not reduced, suggesting a rebound effect. The GWP tends to increase in correlation with the higher plastic waste amount. In contrast, other environmental impacts, like eutrophication, abiotic and acidification potential, are identified to be mitigated effectively via recovery, suppressing the adverse effects of an increase in plastic waste generation. The five-year interval data analysis identified distinct clusters within a set of patterns, categorising them based on their similarities. The categorisation and managerial insights serve as a foundation for devising a focused mitigation strategy.

Post COVID-19 ENERGY sustainability and carbon emissions neutrality.

This review covers the recent advancements in selected emerging energy sectors, emphasising carbon emission neutrality and energy sustainability in the post-COVID-19 era. It benefited from the latest development reported in the Virtual Special Issue of ENERGY dedicated to the 6th International Conference on Low Carbon Asia and Beyond (ICLCA'20) and the 4th Sustainable Process Integration Laboratory Scientific Conference (SPIL'20). As nations bind together to tackle global climate change, one of the urgent needs is the energy sector's transition from fossil-fuel reliant to a more sustainable carbon-free solution. Recent progress shows that advancement in energy efficiency modelling of components and energy systems has greatly facilitated the development of more complex and efficient energy systems. The scope of energy system modelling can be based on temporal, spatial and technical resolutions. The emergence of novel materials such as MXene, metal-organic framework and flexible phase change materials have shown promising energy conversion efficiency. The integration of the internet of things (IoT) with an energy storage system and renewable energy supplies has led to the development of a smart energy system that effectively connects the power producer and end-users, thereby allowing more efficient management of energy flow and consumption. The future smart energy system has been redefined to include all energy sectors via a cross-sectoral integration approach, paving the way for the greater utilization of renewable energy. This review highlights that energy system efficiency and sustainability can be improved via innovations in smart energy systems, novel energy materials and low carbon technologies. Their impacts on the environment, resource availability and social well-being need to be holistically considered and supported by diverse solutions, in alignment with the sustainable development goal of Affordable and Clean Energy (SDG 7) and other related SDGs (1, 8, 9, 11,13,15 and 17), as put forth by the United Nations.

Policy-driven municipal solid waste management assessment using relative quadrant eco-efficiency: A case study in Malaysia.

Despite studies encouraging sustainable waste management, most municipal wastes remain in landfills, particularly in developing countries. Lack of holistic planning and national policy alignment might impair the waste management facility implementation. Policy-driven waste treatment scenarios should be designed to strongly link to the local conditions when assessing the eco-efficiency impacts of the waste management system. Taking Malaysia as a case study, a relative quadrant life cycle eco-efficiency indicator is developed to investigate the eco-efficiency of waste treatment scenarios. The relative quadrant life cycle eco-efficiency indicator depicts the eco-efficiency of various waste management scenarios. Compared with Scenario S1 - business-as-usual (i.e., 71.5% open landfill, 10% sanitary landfill, 1% composting, 17.5% recycling), five waste treatment scenarios (S2-S6) are designed based on Malaysia's existing and future policy targets. Scenario S5 (15.5% sanitary landfill, 22.25% composting, 22.25% anaerobic digestion, 40% recycling) and Scenario S6 (5% sanitary landfill, 22.25% composting, 22.25% anaerobic digestion, 40% recycling, 10.5% incineration) demonstrate that the 40% recycling rate is 32.9-33.6 times more environmentally favorable and 10-20% more economically viable than business-as-usual. Another four scenarios (NS1-NS4) are designed to investigate zero waste in landfills and the need to implement incineration or material recovery. Scenario NS3 suggests increasing incineration capacity to 33% could be an option should incineration is implemented. Adopting home or centralized windrow composting and increasing 2.5-5.5 times of current Feed-in Tariff rates are recommended to improve the eco-efficiency of the waste treatment scenarios. This study could facilitate policymakers to set waste minimization targets and incentives through various scenarios via sensitivity and comparative analyses.

Enhanced precipitation performance for treating high-phosphorus wastewater using novel magnetic seeds from coal fly ash.

Magnetic coagulation is a promising approach for treating high phosphorous (high-P) wastewater by enhancing precipitation efficiency using magnetic particles. In this study, a cost-effective and environmentally friendly magnetic seed from coal fly ash (MS-CFA) was used as an alternative material for Fe3O4 magnetic seed (MS) coagulation. The potential effect of MS-CFA was explored to reduce the settling time and the dosage of coagulant aid of polyacrylamide (PAM) in treating high-phosphorous (high-P) simulated wastewater at 100 and 200 mg P/L. The physicochemical characteristics of MS-CFA were analysed through particle size distribution (20-100 µm), pore size distribution (14-30 nm), specific surface area (1.654 m2/g), X-ray diffraction (XRD), specific gravity (4.2), and magnetic induction intensity (49.8 emu/g). The characteristics met the requirements as magnetic coagulation material. MS-CFA was combined with polyaluminum chloride (PAC) and polyacrylamide (PAM) to improve phosphorous precipitation performance. The synergised magnetic coagulation effect using MS-CFA and PAM reduced the settling time of flocs to less than 1 min due to the high specific gravity. This represents a reduction of 90% of the settling time compared to the control using PAM alone (15 min) without MS-CFA. MS-CFA efficiently reduced PAM dosage by 83% and 87% for treating 100 and 200 mg P/L, respectively. The presence of PAM (1 mg/L for 100 mg P/L and 2 mg/L for 200 mg P/L) was imperative for binding the MS-CFA and flocs, hence increasing the particle size of the magnetic flocs. The characteristics of the magnetic flocs were analysed through microscopy, particle size distribution, zeta potential measurements, and magnetic induction intensity. The characteristics of the magnetic flocs confirmed that MS-CFA could be an alternative material for Fe3O4 as the magnetic seeds in the magnetic coagulation process for treating high-P wastewater.

Forecasting plastic waste generation and interventions for environmental hazard mitigation.

Plastic waste and its environmental hazards have been attracting public attention as a global sustainability issue. This study builds a neural network model to forecast plastic waste generation of the EU-27 in 2030 and evaluates how the interventions could mitigate the adverse impact of plastic waste on the environment. The black-box model is interpreted using SHapley Additive exPlanations (SHAP) for managerial insights. The dependence on predictors (i.e., energy consumption, circular material use rate, economic complexity index, population, and real gross domestic product) and their interactions are discussed. The projected plastic waste generation of the EU-27 is estimated to reach 17 Mt/y in 2030. With an EU targeted recycling rate (55%) in 2030, the environmental impacts would still be higher than in 2018, especially global warming potential and plastic marine pollution. This result highlights the importance of plastic waste reduction, especially for the clustering algorithm-based grouped countries with a high amount of untreated plastic waste per capita. Compared to the other assessed scenarios, Scenario 4 with waste reduction (50% recycling, 47.6% energy recovery, 2.4% landfill) shows the lowest impact in acidification, eutrophication, marine aquatic toxicity, plastic marine pollution, and abiotic depletion. However, the global warming potential (8.78 Gt CO2eq) is higher than that in 2018, while Scenario 3 (55% recycling, 42.6% energy recovery, 2.4% landfill) is better in this aspect than Scenario 4. This comprehensive analysis provides pertinent insights into policy interventions towards environmental hazard mitigation.

Dilution rate of compost leachate from different biowaste for the fertigation of vegetables.

Compost leachate (CL) is often treated as sewage. Limited studies have characterised the use of CL for agriculture application due to the variation of nutrient range, potential pollutants and need for pretreatment before use. This paper reviewed the characteristics of CL in terms of its nutrients and physical characteristics from three types of biowastes, i.e. the organic portion of municipal solid waste (OP-MSW), animal manure and green waste. The nutrient range characterised are used to develop the dilution rate of the CL as fertigation water for three types of vegetables. The dilution rate of CL developed is based on the fertigation standards set by the Food and Agriculture Organization of the United Nations and Malaysia Standards. The results show that all CL contain high organic content and micronutrients, which exceeded the upper limits. For the fertigation of vegetables using the CL, based on the requirement of COD and micronutrients (Fe, Mn, Zn, Cu, Pb, Ni, Cd, Cr) of the vegetables, the CL from green waste required the lowest dilution rate (8-45 fold), followed by animal waste (33-65 fold) and the OP-MSW (193-1770 fold). The novel dilution range developed is vital to balance the essential nutrients in the CL while avoiding phototoxicity on plant and soil pollution. The dilution strategy developed is essential to support the conversion of CL as an organic liquid fertiliser for agricultural application.

Uncovering the dynamics in global carbon dioxide utilization research: a bibliometric analysis (1995-2019).

The anthropogenic emission of carbon dioxide (CO2) into the atmosphere is recognized as the main contributor to global climate change. To date, scientists have developed various strategies, including CO2 utilization technologies, to reduce global carbon emissions. This paper presents the global scientific landscape of the CO2 utilization research from 1995 to 2019 based on a bibliometric analysis of 1875 publications extracted from Web of Science. The findings indicate a major increase in the number of publications and citations received from 2015 to 2019, denoting a fast-emerging research trend. The dynamics of global CO2 utilization research is partly driven by China's policies and research funding to promote low-carbon economic development. Applied Energy is recognized as a core journal in this research topic. The utilization of CO2 is a multidisciplinary topic that has progressed by multidimensional collaborations at the country and organizations levels, while the formation of co-authorship networks at the individual level is mostly influenced by the authors' affiliations. Keyword co-occurrence analysis reveals a rapid evolution in the CO2 utilization strategies from chemical fixation in carbonates and epoxides to pilot-scale testing of power-to-gas technologies in Europe and the USA. The development of efficient power-to-fuel technologies and biological utilization routes (using microalgae and bacteria) will probably be the next research priorities in CO2 utilization research.

Microplastics and nanoplastics in global food webs: A bibliometric analysis (2009-2019).

This paper presents the research landscape on microplastics and nanoplastics (M/NPs) in global food webs based on a bibliometric analysis of 330 publications published in 2009-2019 extracted from Web of Science. The publications increased tremendously since 2013. Marine Pollution Bulletin is one of the top productive journals for this topic. The publication landscape related to M/NPs in global food webs, as interdisciplinary research, is highly dependent on the funding availability. The high productivities of England, China, USA and European countries are attributed to the funding from the agencies at regional or national levels. Keyword analysis reveals the shift of research hotspots from investigations on M/NPs absorbed by various organisms in the ecosystems to studies on the trophic transfer of M/NPs and sorbed contaminants in the food webs and their associated adverse impacts. Funding agencies play important roles in leading the future development of this topic.

Effect of aquaculture salinity on nitrification and microbial community in moving bed bioreactors with immobilized microbial granules.

The novel immobilized microbial granules (IMG) shows a significant effect of nitrification for freshwater aquaculture. However, there is lack of evaluation study on the performance of nitrification at high salinity due to the concentration of recycled water or seawater utilization. A laboratory scale moving bed bioreactor (MBBR) with IMG was tested on recycled synthetic aquaculture wastewater for the nitrification at 2.5 mg/L NH3-N daily. The results indicated that IMG showed a high salinity tolerance and effectively converted ammonia to nitrate up to 92% at high salinity of 35.0 g/L NaCl. As salinity increased from near zero to 35.0 g/L, the microbial activity of nitrite oxidation bacteria (NOB) in the IMG decreased by 86.32%. The microbial community analysis indicated that salinity significantly influenced the community structure. It was found that Nitrosomonas sp. and Nitrospira sp. were the dominant genera for ammonia oxidation bacteria (AOB) and NOB respectively at different salinity levels.

Environmental and economic feasibility of an integrated community composting plant and organic farm in Malaysia.

Waste prevention and management become a significant issue worldwide to achieve sustainable development. Similar to many developing countries, Malaysia has faced severe problems in waste management due to its rapid economic growth and urbanisation. The municipal solid waste (MSW) production rate in Malaysia had increased significantly in a recent year, ranging from 0.8 to 1.25 kg/person∙d. The wastes generated contain a high amount of organic portion with high moisture content. Improper MSW management practice or delayed in waste collection and transportation can lead to severe health issues. This paper presents a case study in Johor Bahru, Malaysia (FOLO Farm), in which a composting prototype is used as the waste management technology to recycle the food and vegetable wastes. The greenhouse gases (GHG) mitigation and economic feasibility of the integrated composting and organic farming in this study are reported. This study showed a reduction of 27% of GHG by diverting the food and vegetable wastes from open dumping to the composting plant. Higher reduction rate (∼44%) can be achieved with better planning of waste collection route and applying the mitigation strategies during the composting process. By adapting the membership concept, this project not only ensures the economic feasibility of running a composting plant but also secures a channel for the growth of vegetable distribution. This study provides an insight into the feasibility and desirability to implement a pilot-scale composting for organic waste management to achieve the low carbon and self-sustain community.

Raw oil palm frond leaves as cost-effective substrate for cellulase and xylanase productions by Trichoderma asperellum UC1 under solid-state fermentation.

Production of cellulases and xylanase by a novel Trichoderma asperellum UC1 (GenBank accession no. MF774876) under solid state fermentation (SSF) of raw oil palm frond leaves (OPFL) was optimized. Under optimum fermentation parameters (30 °C, 60-80% moisture content, 2.5 × 106 spores/g inoculum size) maximum CMCase, FPase, ß-glucosidase and xylanase activity were recorded at 136.16 IU/g, 26.03 U/g, 130.09 IU/g and 255.01 U/g, respectively. Cellulases and xylanase were produced between a broad pH range of pH 6.0-12.0. The enzyme complex that comprised of four endo-ß-1,4-xylanases and endoglucanases, alongside exoglucanase and ß-glucosidase showed thermophilic and acidophilic characteristics at 50-60 °C and pH 3.0-4.0, respectively. Glucose (16.87 mg/g) and fructose (18.09 mg/g) were among the dominant sugar products from the in situ hydrolysis of OPFL, aside from cellobiose (105.92 mg/g) and xylose (1.08 mg/g). Thermal and pH stability tests revealed that enzymes CMCase, FPase, ß-glucosidase and xylanase retained 50% residual activities for up to 15.18, 4.06, 17.47 and 15.16 h of incubation at 60 °C, as well as 64.59, 25.14, 68.59 and 19.20 h at pH 4.0, respectively. Based on the findings, it appeared that the unique polymeric structure of raw OPFL favored cellulases and xylanase productions.

Anaerobic digestion of lignocellulosic waste: Environmental impact and economic assessment.

Lignocellulosic waste (LW) is abundant in availability and is one of the suitable substrates for anaerobic digestion (AD). However, it is a complex solid substrate matrix that hinders the hydrolysis stage of anaerobic digestion. This study assessed various pre-treatment and post-treatments of lignocellulosic waste for anaerobic digestion benefiting from advanced P-graph and GaBi software (Thinkstep, Germany) from the perspective of cost and environmental performances (global warming potential, human toxicity, ozone depletion potential, particulate matter, photochemical oxidant creation, acidification and eutrophication potential). CaO pre-treatment (P4), H2S removal with membrane separation post-treatment (HSR MS) and without the composting of digestate is identified as the cost-optimal pathway. The biological (P7- Enzyme, P8- Microbial Consortium) and physical (P1- Grinding, P2- Steam Explosion, P3- Water Vapour) pre-treatments alternatives have lower environmental impacts than chemical pre-treatments (P4- CaO, P5- NaOH, P6- H2SO4) however they are not part of the near cost optimal solutions. For post-treatment, the near cost optimal alternatives are H2S removal with organic physical scrubbing (HSR OPS) and H2S removal with amine scrubbing (HSR AS). HSR AS has a better performance in the overall environmental impacts followed by HSR MS and HSR OPS. In general, the suggested cost-optimal solution is still having relatively lower environmental impacts and feasible for implementation (cost effective). There is very complicated to find a universal AD solution. Different scenarios (the type of substrate, the scale, product demand, policies) have different constraints and consequently solutions. The trade-offs between cost and environment performances should be a future extension of this work.

Anaerobic digestion of municipal solid waste: Energy and carbon emission footprint.

Anaerobic digestion (AD) serves as a promising alternative for waste treatment and a potential solution to improve the energy supply security. The feasibility of AD has been proven in some of the technologically and agriculturally advanced countries. However, development is still needed for worldwide implementation, especially for AD process dealing with municipal solid waste (MSW). This paper reviews various approaches and stages in the AD of MSW, which used to optimise the biogas production and quality. The assessed stages include pre-treatment, digestion process, post-treatment as well as the waste collection and transportation. The latest approaches and integrated system to improve the AD process are also presented. The stages were assessed in a relatively quantitative manner. The range of energy requirement, carbon emission footprint and the percentage of enhancement are summarised. Thermal hydrolysis pre-treatment is identified to be less suitable for MSW (-5% to +15.4% enhancement), unless conducted in the two-phase AD system. Microwave pre-treatment shows consistent performance in elevating the biogas production of MSW, but the energy consumption (114.24-8,040 kWeh t-1) and carbon emission footprint (59.93-4,217.78 kg CO2 t-1 waste) are relatively high. Chemical (∼0.43 kWeh m-3) and membrane-based (∼0.45 kWeh m-3) post-treatments are suggested to be a lower energy consumption approach for upgrading the biogas. The feasibility in terms of cost (scale up) and other environmental impacts (non-CO2 footprint) needs to be further assessed. This study provides an overview to facilitate further development and extended implementation of AD.

Effects of different vermicompost extracts of palm oil mill effluent and palm-pressed fiber mixture on seed germination of mung bean and its relative toxicity.

Several treatment technologies are available for the treatment of palm oil mill wastes. Vermicomposting is widely recognized as efficient, eco-friendly methods for converting organic waste materials to valuable products. This study evaluates the effect of different vermicompost extracts obtained from palm oil mill effluent (POME) and palm-pressed fiber (PPF) mixtures on the germination, growth, relative toxicity, and photosynthetic pigments of mung beans (Vigna radiata) plant. POME contains valuable nutrients and can be used as a liquid fertilizer for fertigation. Mung bean seeds were sown in petri dishes irrigated with different dilutions of vermicomposted POME-PPF extracts, namely 50, 60, and 70% at varying dilutions. Results showed that at lower dilutions, the vermicompost extracts showed favorable effects on seed germination, seedling growth, and total chlorophyll content in mung bean seedlings, but at higher dilutions, they showed inhibitory effects. The carotenoid contents also decreased with increased dilutions of POME-PPF. This study recommends that the extracts could serve as a good source of fertilizer for the germination and growth enhancement of mung bean seedlings at the recommended dilutions.

Evaluation of Effective Microorganisms on home scale organic waste composting.

Home composting can be an effective way to reduce the volume of municipal solid waste. The aim of this study is to evaluate the effect of Effective Microorganism™ (EM) for the home scale co-composting of food waste, rice bran and dried leaves. A general consensus is lacking regarding the efficiency of inoculation composting. Home scale composting was carried out with and without EM (control) to identify the roles of EM. The composting parameters for both trials showed a similar trend of changes during the decomposition. As assayed by Fourier Transform Infrared Spectroscopy (FTIR), the functional group of humic acid was initially dominated by aliphatic structure but was dominated by the aromatic in the final compost. The EM compost has a sharper peak of aromatic CC bond presenting a better degree of humification. Compost with EM achieved a slightly higher temperature at the early stage, with foul odour suppressed, enhanced humification process and a greater fat reduction (73%). No significant difference was found for the final composts inoculated with and without EM. The properties included pH (∼7), electric conductivity (∼2), carbon-to-nitrogen ratio (C: N < 14), colour (dark brown), odour (earthy smell), germination index (>100%), humic acid content (4.5-4.8%) and pathogen content (no Salmonella, <1000 Most Probable Number/g E. coli). All samples were well matured within 2 months. The potassium and phosphate contents in both cases were similar however the EM compost has a higher nitrogen content (+1.5%). The overall results suggested the positive effect provided by EM notably in odour control and humification.

Towards low carbon society in Iskandar Malaysia: Implementation and feasibility of community organic waste composting.

Rapid population growth and urbanisation have generated large amount of municipal solid waste (MSW) in many cities. Up to 40-60% of Malaysia's MSW is reported to be food waste where such waste is highly putrescible and can cause bad odour and public health issue if its disposal is delayed. In this study, the implementation of community composting in a village within Iskandar Malaysia is presented as a case study to showcase effective MSW management and mitigation of GHG emission. The selected village, Felda Taib Andak (FTA), is located within a palm oil plantation and a crude palm oil processing mill. This project showcases a community-composting prototype to compost food and oil palm wastes into high quality compost. The objective of this article is to highlight the economic and environment impacts of a community-based composting project to the key stakeholders in the community, including residents, oil palm plantation owners and palm oil mill operators by comparing three different scenarios, through a life cycle approach, in terms of the greenhouse gas emission and cost benefit analysis. First scenario is the baseline case, where all the domestic waste is sent to landfill site. In the second scenario, a small-scale centralised composting project was implemented. In the third scenario, the data obtained from Scenario 2 was used to do a projection on the GHG emission and costing analysis for a pilot-scale centralised composting plant. The study showed a reduction potential of 71.64% on GHG emission through the diversion of food waste from landfill, compost utilisation and significant revenue from the compost sale in Scenario 3. This thus provided better insight into the feasibility and desirability in implementing a pilot-scale centralised composting plant for a sub-urban community in Malaysia to achieve a low carbon and self-sustainable society, in terms of environment and economic aspects.

Physicochemical profile of microbial-assisted composting on empty fruit bunches of oil palm trees.

This study was carried out to investigate the physicochemical properties of compost from oil palm empty fruit bunches (EFB) inoculated with effective microorganisms (EM∙1™). The duration of microbial-assisted composting was shorter (∼7 days) than control samples (2 months) in a laboratory scale (2 kg) experiment. The temperature profile of EFB compost fluctuated between 26 and 52 °C without the presence of consistent thermophilic phase. The pH of compost changed from weak acidic (pH ∼5) to mild alkaline (pH ∼8) because of the formation of nitrogenous ions such as ammonium (NH4 (+)), nitrite (NO2 (-)), and nitrate (NO3 (-)) from organic substances during mineralization. The pH of the microbial-treated compost was less than 8.5 which is important to prevent the loss of nitrogen as ammonia gas in a strong alkaline condition. Similarly, carbon mineralization could be determined by measuring CO2 emission. The microbial-treated compost could maintain longer period (∼13 days) of high CO2 emission resulted from high microbial activity and reached the threshold value (120 mg CO2-C kg(-1) day(-1)) for compost maturity earlier (7 days). Microbial-treated compost slightly improved the content of minerals such as Mg, K, Ca, and B, as well as key metabolite, 5-aminolevulinic acid for plant growth at the maturity stage of compost. Graphical Abstract Microbial-assisted composting on empty fruit bunches.

Edible Bird's nest extract as a chondro-protective agent for human chondrocytes isolated from osteoarthritic knee: in vitro study.

BACKGROUND: Osteoarthritis (OA) is a degenerative joint disease that results in the destruction of cartilage. Edible Bird's Nest (EBN) extract contains important components, which can reduce the progression of osteoarthritis and helps in the regeneration of the cartilage. The present study aimed to investigate the effect of EBN extract on the catabolic and anabolic activities of the human articular chondrocytes (HACs) isolated from the knee joint of patients with OA. METHODS: A single batch of EBN extract was prepared with hot-water extraction and coded as HMG. HACs were isolated from the knee joint cartilage removed during surgery. The optimum concentration of HMG for HAC cultures was determined using MTT assay. The effect of HMG on the catabolic and anabolic genes' expression in HACs was measured by real-time PCR. The total amount of prostaglandin E2 (PGE2) production was determined by ELISA method, and the total sulphated glycosaminoglycan (GAGs) production was quantified by 1,9-dimethylmethylene blue (DMMB) assay. RESULTS: MTT assay showed 0.50% - 1.00% HMG supplementation promoted HACs proliferation. HMG supplementation was able to reduce the catabolic genes' expression in cultured HACs such as matrix metalloproteinases (MMP1 & MMP3), Interleukin 1, 6 and 8 (IL-1, IL-6 & IL-8), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). Prostaglandin E2 (PGE2) production was significantly reduced in HAC cultures supplemented with HMG. With regard to anabolic activity assessment, type II collagen, Aggrecan and SOX-9 gene expression as well as sGAG production was increased in the HMG supplemented groups. CONCLUSION: Edible Bird's Nest extract coded as HMG demonstrated chondro-protection ability on human articular chondrocytes in vitro. It reduced catabolic activities and increased cartilage extracellular matrix synthesis. It is concluded that HMG is a potential agent in the treatment of osteoarthritis.